No traces of coronavirus were found in tests at four major railway stations and on intercity train services, Network Rail said.
Places passengers regularly touch such as escalator handles, ticket machines, and benches were swabbed, while hour-long air samples were taken.
Two rounds of testing were carried out at London Euston, Birmingham New Street, Liverpool Lime Street, and Manchester Piccadilly station, in January and June.
Tests were repeated on trains running between the stations.
Examination of the results by Imperial College London found no COVID-19 contamination of any surface, or airborne particles of the virus.
Rob Mole, senior programme manager for Network Rail’s response to the pandemic, said: “Station cleaning teams and train staff have made it their mission to keep passengers safe during the pandemic and this is proof their dedicated approach works.
“We want all passengers to travel in confidence on the railway network and we will keep doing our part by rigorously cleaning trains and stations. – READ MORE